Study pollen grains in rice by using multispectral imaging techniques

摘要

This paper describes a novel multispectral imaging microscope and its applications in the study of pollen grains in rice.The Imaging instruments can simultaneously record both spectral and spatial information of a sample, which is helpfulto study the chemical states and physical properties of the sample by taking advantage of spatial image processing andspectroscopic analysis techniques. A LCTF (liquid crystal tunable filter) device is used for fast wavelength selection inthe range of 400nm to 720nm and a cooled two-dimensional monochrome CCD for image detection. In this paper, theimage acquisition process, spatial and spectral calibration and spectral imaging analysis methods are detailed. And alsoa novel method using this multispectral imaging microscope to observe rice pollen grains is reported here. Themultispectral images were systematically processed and analyzed by the software. The results illustrated that thetransmittance analysis of multispectral pollen images can accurately identify the pollen abortion stage of male-sterilerice, and can easily distinguish a variety of male sterile cytoplasm. Compared with cytological and histochemicalmethods reported previously, the method reported here has demonstrated to be more efficient and reliable in the study ofchemical states and physical properties in plant cells.