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Study pollen grains in rice by using multispectral imaging techniques

机译:利用多光谱成像技术研究水稻中的花粉粒

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摘要

This paper describes a novel multispectral imaging microscope and its applications in the study of pollen grains in rice. The Imaging instruments can simultaneously record both spectral and spatial information of a sample, which is helpful to study the chemical states and physical properties of the sample by taking advantage of spatial image processing and spectroscopic analysis techniques. A LCTF (liquid crystal tunable filter) device is used for fast wavelength selection in the range of 400nm to 720nm and a cooled two-dimensional monochrome CCD for image detection. In this paper, the image acquisition process, spatial and spectral calibration and spectral imaging analysis methods are detailed. And also a novel method using this multispectral imaging microscope to observe rice pollen grains is reported here. The multispectral images were systematically processed and analyzed by the software. The results illustrated that the transmittance analysis of multispectral pollen images can accurately identify the pollen abortion stage of male-sterile rice, and can easily distinguish a variety of male sterile cytoplasm. Compared with cytological and histochemical methods reported previously, the method reported here has demonstrated to be more efficient and reliable in the study of chemical states and physical properties in plant cells.
机译:本文介绍了一种新型的多光谱成像显微镜及其在水稻花粉粒研究中的应用。成像仪器可以同时记录样品的光谱和空间信息,这有助于利用空间图像处理和光谱分析技术来研究样品的化学状态和物理性质。 LCTF(液晶可调滤光片)设备用于在400nm至720nm范围内进行快速波长选择,并使用冷却的二维单色CCD进行图像检测。本文详细介绍了图像采集过程,空间和光谱校准以及光谱成像分析方法。此外,这里还报道了使用这种多光谱成像显微镜观察水稻花粉粒的新方法。该软件对多光谱图像进行了系统处理和分析。结果表明,多光谱花粉图像的透射率分析可以准确地识别雄性不育水稻的花粉流产阶段,并可以轻松地区分多种雄性不育细胞质。与先前报道的细胞学和组织化学方法相比,此处报道的方法在研究植物细胞的化学状态和物理特性方面已被证明更加有效和可靠。

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