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METHOD OF STUDY OF MORPHOLOGY OF GRAINS POLLEN OF PLANTS

机译:植物籽粒花粉形态的研究方法

摘要

FIELD: agriculture.;SUBSTANCE: invention is a method of assessment of morphology of the pollen grains of plants, consisting in their staining and induction of fluorescence in incident light using the inverted fluorescence microscope, characterized in that the preparation of pollen on specimen glass is treated with a complex dye consisting of 20 parts by volume of 0.00001% aqueous solution of Hoechst 33258 and 1 part of 2% alcoholic solution of pyronine B, and epi-luminescence of the stained pollen grains is studied, excited by the incident light in the wavelength range of 340-380 nm, with a locking optical filter transmitting long-wave range of spectrum of more than 510 nm.;EFFECT: invention enables to accelerate evaluation of the morphology of pollen grains, the size and nature of location of their apertures on contrast images obtained.;3 dwg, 3 ex
机译:技术领域:本发明是一种评估植物花粉粒形态的方法,其包括使用倒置荧光显微镜对植物的花粉进行染色和在入射光中诱导荧光,其特征在于,在标本玻璃上制备花粉的方法是:用由20体积份的0.00001%的Hoechst 33258水溶液和1份的2%的吡咯烷B的醇溶液组成的复合染料处理,并研究了被污染的花粉颗粒的落射荧光,并由入射光激发波长范围为340-380 nm,带有锁定滤光片,可透射超过510 nm的长波光谱。效果:本发明可以加快对花粉粒形态,孔径大小和位置性质的评估在获得的对比图像上; 3 dwg,3前

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