Effect of Surface Chemistry on Stem Cell Response in 2-D vs. 3-D Cell Culture Niches

摘要

We have successfully developed a material system that enables the systematic investigation of the effects of scaffold structure and chemistry in 2-D vs. 3-D cultures. The results show that PCL surface hydrolysis chemistry affects cell shape and differentiation in 2-D culture but not in 3-D culture, indicating that the surface chemistry has less effect on cell function in 3-D than in 2-D.