AN ALTERNATIVE METHODOLOGY FOR A QUANTITATIVE FLOW-BASED CELL-MEDIATED IN VITRO CYTOTOXICITY ASSAY TO EVALUATE IMMUNE CELL POTENCY

摘要

In vitro cytotoxicity assays have a crucial role in determining the functionality and potency levels of various immune cells, including both NK and T-cells. A flow cytometry based cytoxicity assay is commonly used to measure the levels of cell-mediated killing of a target cancer cell. The assay involves staining the cancer cells along with a co-culture incubation step. The cytotoxicity value is typically reported as a percentage killing efficiency of the target cancer cell at a fixed effector-to-target ratio. In vitro cytotoxicity assays have commonly been used to provide valuable potency data and have many diverse applications for multiple cell types.