Optimisation of brewing yeast differentiation andwild yeast identification by Real-Time PCR

摘要

The differentiation of brewing yeasts and the detection of wild yeasts based on cultivationrnoften causes difficulties because of high similarities amongst brewing yeasts and betweenrnbrewing and wild yeasts respectively. To get a more rapid and specific detection of thesernorganisms different Real-Time PCR methods were developed. Using Real-Time PCR it wasrnpossible to establish assays for the differentiation of bottom-and top-fermenting yeasts.rnMoreover specific assays were designed for the detection of harmful wild yeasts, e.g. S.rnDiastaticus, Dekkera spp. Combining PCR with selective cultivation (MYPG+CuSOrnCuSO4) preexistingrnknow-how was successfully combined with PCR and identification accuracy wasrnimproved.