The differentiation of brewing yeasts and the detection of wild yeasts based on cultivation often causes difficulties because of high similarities amongst brewing yeasts and between brewing and wild yeasts respectively. To get a more rapid and specific detection of these organisms different Real-Time PCR methods were developed. Using Real-Time PCR it was possible to establish assays for the differentiation of bottom-and top-fermenting yeasts. Moreover specific assays were designed for the detection of harmful wild yeasts, e.g. S. Diastaticus, Dekkera spp. Combining PCR with selective cultivation (MYPG+CuSO CuSO4) preexisting know-how was successfully combined with PCR and identification accuracy was improved.
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机译：酿造酵母的分化和基于培养的野生酵母的检测通常由于酿造酵母和酿造和野生酵母之间的高相似性而导致困难。为了更快地检测这些生物体，产生不同的实时PCR方法。使用实时PCR，可以建立用于分化的底部和顶部发酵酵母的测定。此外，专门的测定是为了检测有害的野生酵母，例如， S. diakerativeS，Dekkera SPP。将PCR与选择性培养（Mypg + Cuso Cuso4）相结合，先前发布的知识与PCR成功结合PCR和鉴定准确性得到了改善。
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