Subcellular distribution of photodynamic sensitizers

摘要

Abstract: Various microscopic methods were established to measure the subcellular distribution and light-induced reactions of photosensitizing porphyrins. These methods include high resolution video microscopy, microspectrofluorometry, as well as time-resolved and time-gated fluorescence spectroscopy. By using these techniques, different components of hydrophylic and hydrophobic photosensitizers (e.g. monomers, aggregates, ionic species) were localized, and their intracellular reactions - re-distribution, formation of photoproducts, pH-dependent spectral changes - were studied. !14