The characteristic research of absorption spectrum about ALA

摘要

The characteristics of ALA absorption spectrum were studied by comparing the difference of ALA absorption spectrum in different conditions in the paper. The results of test show that the wavelength of the strongest absorption spectrum of ALA is 270nm. The relationship between the serum solution density and the absorption peak value is the inverse ratio. The obstructive effect will be more obvious when the density of serum is thicker. The best serum solution density is 10%. The amount of PpIX will be the biggest when the incubate time is suitable and the right time is 8 hours for Jurkat cell.