UV-A fiber-optic technique of the local function injury of the bundle of axons using radiation of N_2 laser (λ = 337 nm)

摘要

The method of the local (>200 μm) functional injury of somatic frog's nerve using UV-A radiation of N_2 laser (λ= 337nm, P ～ 10-100 mW) was realized. The level of injury was assessed from the decrease of compound action potential of the nerve stimulated extracellularly by electrical pulses of millisecond duration. Study of dynamic response of the bundle of axons revealed the decrease of the number of action potential which can be generated (less than 10~5). This decrease may be attributed to destruction of action transport or/and voltage-activated ion channels of axon membrane. The dose dependence of the level of nerve injury was studied using varied both intensity and duration of UV-A irradiation.