Technology and mechanism of neuron inactivation by N_2 laser radiation ( λ =337 nm), mercury-vapor lamp (Hg λ=365 nm) and low-coherence UV-A sun radiation

摘要

The method of the local functional injury of somatic frog's nerve using high coherent UV-A radiation of N_2 laser(λ= 337nm , P ～ 10 mW), coherent mercury-vapor lamp radiation (λ= 365; 312 nm P_∑ ～ 2 mW/cm~2) and low-coherence UV-A sun radiation (400-315 nm, P_∑ ～ 10 mW ) was realized. The level of injury was assessed from the decrease of compound action potential of the nerve stimulated extracellularly by electrical pulses of millisecond duration. Study of dynamic response of the bundle of axons revealed the decrease of the number of action potential which can be generated (less than 10~5). This decrease may be attributed to destruction of action transport or/and voltage-activated ion channels of axon membrane. The dose dependence of the level of nerve injury was studied using varied both intensity and duration of high and low- coherent UV-A irradiation.