INTERACTION OF NUCLEIC ACIDS AND LIPIDS FROM TUMOUR CELLS WITH ANTICANCER DRUGS: AN SEIRA SPECTROSCOPY DATA

摘要

It is known that malignant tumour progression in the direction of the emergence of tumour drug resistance is one of the main factors resulting in the failure of cancer treatment. As tumour progression is based on the genetic instability, the study of the structure of nucleic acid from tumour cells is of great importance both for basic knowledge and biomedical application. We applied surface enhanced infrared spectroscopy (SEIRA) of nucleic acids on gold substrate and essentially increased the sensitivity of IR spectroscopy. We observed numerous changes in infrared spectra of DNA from sensitive and resistant cells that reflect drastic changes in molecular structure of DNA from tumour cells. The DNA from resistant cancer cells could be characterised as rigid structure, the structure of DNA from sensitive cancer strain seems to be flexible and after application of anticancer drugs drastically changes and approaches to the structure of helix forms. The molecular structure of lipids from resistant and sensitive cancers after application of anti-tumour drugs is modified also. Thus, we observed a disordering in the lipid chain packing from resistant cells after application of cisplatin and formation of phospholipid-Pt complex in some cases.