On the influence of cell parameters in environmental stress detection by Fourier Transform Infrared Spectroscopy analysis of whole yeast cells.

摘要

Fourier Transform InfraRed (FTIR) spectroscopy is an increasingly used technique in biology, especially for whole cell metabolomic fingerprint (Forrester et al, 2009; Yu et al, 2009). The reproducibility of this technique is influenced by a large number of factors such as the physiological state of cells, sample manipulation and growth conditions. Extrinsic fonts of variations are the growth media and the growth phase of the microbial culture (Meade et al, 2007; Oust et al, 2004). Cell shape and dimension can be further elements to consider in whole cell FTIR analysis (Rebuffo-Scheer et al, 2007). In order to address the effect of cell geometry on the FTIR spectra and to define the extent of variability occurring between machine and biological replicas with a standardized protocol two yeast species were employed for their different morphology: Saccharomyces cerevisiae (large oval-shaped cells) and Debaryomyces hansenii (small round shaped cells).