Adaptive optics in wide-field microscopy

摘要

Three-dimensional live imaging in cell biology is hindered by optical aberrations which degrade the resolution and signal-to-noise ratio as the focal plane is moved deeper into the sample. The solution to this problem is to use adaptive optics to correct the aberrations. In this paper, we discuss our work on applying adaptive optics to wide-field fluorescence microscopy. We demonstrate correction of depth-aberrations and focusing using a deformable mirror in open-loop operation. We then discuss the use of phase retrieval and phase diversity in adaptive optics.