(Keynote) Electrochemical Interface Using a New Double Mediator System for Intracellular NADH Sensing and Acute Cytotoxicity Test

摘要

Nicotinamide Adenine Dinucleotide (reduced form), NADH is a very important redox compound that is essential for metabolic reactions and ATP production in all living cells. Intracellular NADH metabolism is also important to consider the cancer treatment. Intracellular NADH has been conventionally measured by WST assay using a cell membrane-permeable redox mediator, 1-methoxy PMS (1-Methoxy-5-methylphenazinium methylsulfate) and a water soluble tetrazolium to form formazan. This colorimetric method is very easy and useful for cell counting and cytotoxicity test. However, WST assay is time-consuming and then it is not able to apply for evaluation of acute cytotoxicity by chemical compounds including drugs and pollutants. On the other hand, some research groups have already shown that electrochemical method with double mediator system is useful to monitor rapidly intracellular NADH level corresponding to the cell viability.