An efficient aqueous peptide and protein surface immobilization method for use in biomolecular recognition

摘要

Surface plasmon resonance-based biosensors provide a sophisticated and discriminating means of probing biomolecular interactions.Specific ligands such as peptides and proteins can be immobilized onto the sensor surfaces by a number of means including covalent attachment via amine,thiol or aldehyde chemistry,capture via biotin-avidin/neutravidin interaction or the use of specific tags.However,each of these immobilization strategies has advantages and disadvantages.For example,some non-specific binding to neutravidin has been observed(cytoskeletal protein,tubulin,human serum albumin)and found to complicate subsequent interaction analysis as well hamper subsequent downstream mass spectrometry analyses of purified protein complexes.