A useful method for monitoring of peptide synthesis on soluble polymer support

摘要

Polyethylene glycols(PEG)s have been successfully used as polymer soluble supports in the synthesis of peptides,oligosacharides and oligonucleotides,The synthesis is carried out under homogeneous conditions while purification is performed by precipitation of the polymer.They also offer certain advantages over insoluble polymers in terms of analysis and products monitoring because the molecules anchored to soluble PEG can be fully characterized in both the solid and solution state by NMR,IR,CD,Raman spectroscopy.However,quantitative monitoring of the peptide chain growth is advantage during the synthesis.For that reason,we developed new method for monitoring of loading level within synthesis on polymer soluble support.