Phytochemical Screening and Antibacterial Activity of Flower, Stem, and Tuber of Polianthes tuberosa L. Against Acne-Inducing Bacteria

摘要

Objectives: The aim of this study was to determine the antibacterial activity of flower, stem, and tuber of Polianthes tuberosa L against Staphylococcus epidermidis and Propionibacterium acnes. Method: The extraction was conducted by a maceration method using different polarity of solvent (n-hexane, ethyl acetate, and methanol). The antibacterial activity and the minimum inhibitory concentration (MIC) were determined using the agar diffusion method. The analysis identified by thin layer chromatography (TLC), bioauthography assay, and scanning electron microscopy (SEM). Results and Discussion: The antibacterial activity showed the most active extract was a methanol extract of the flower against Staphylococcus epidermidis and Propionibacterium acnes with MIC value 30 mg/ml. Observations using SEM showed that the methanol extract of flower caused to morphological change on a cellular wall of Propionibacterium acnes, and therefore the antibacterial activity was related to damaged the bacterial cellular wall. The result of bioautography assay showed the methanol extract of flower have an antibacterial activity against Staphylococcus epidermidis and Propionibacterium acnes with Rf value at 0.87.. Conclusion: The methanol extract of the Polianthes tuberosa L. flower has antibacterial activity against acne-causing bacteria by causing damage to cell walls.