Gene Transfer in Japanese Flounder (Paralichthys olivaceus) Via Electroporation

摘要

The "all fish" chinook salmon growth hormone gene driven by the antifreeze protein gene(AFP) promoter of opcean pout was introduced into the fertilized eggs of Japanese flounder paralichtys olivaceus via electroporation. Successful transfer of foreign DAN was detected by polymerase chain reaction (PCR). Under optimum conditions, a transfer efficiency of 16.7