The Potential of Yacon Root (Smalanthus sonchifolius (Poepp. et Endl.) H. Robinson) as Prebiotics to Stimulate Growth of Lactobacillus plantarum B1765

摘要

Yacon (Smalanthus sonchifolius Poep p. Endl.) root is a source of inulin and fructooligosacharide (FOS) which potent as a prebiotic. Inulin and FOS hydrolyzed to glucose and fructans by inulinase which is secreted by probiotics bacteria and further metabolized to short chain fatty acids (SCFA) that were known as bioactive compound to prevent some degenerative diseases. This research observed the potential of yacon roots as prebiotics to stimulate the growth of L.plantarum B1765, a probiotic bacteria candidate, for some aspect including the number of lactic acids bacteria (LAB), pH, acidity and the profile of SCFA producing. Enumeration of LAB was done by total plate count using MRS medium + CaCO_3, acidity by titration, and the profile of SCFA were analyzed by HPLC. The growth of L.plantarum B1765 in yacon medium for 48 hours increased from 1,12×10~6 to 3,25×10~8 CFU/g, pH decreased to 3.28, and total acids increased from 0.04% to 0.45%. The yields of SCFA for 7 days of fermentation are propionic acid 1182.30 ppm, acetic acid 803.28 ppm and butyric acid 375.41 ppm. Yacon roots are potential as prebiotics especially for the growth of L.plantarum B1765 as the probiotic candidate.