Production of antiviral compounds against encephalomyocarditis virus (EMCV) from woody biomass by microwave reaction

摘要

Woody biomass is a renewable bio-resource on earth, and was produced in huge amount worldwide. The sustainable resource is still underutilized due to the limited value of the final products. In this research, we developed a method for production of high value-added bioactive compounds from woody biomass by microwave degradation. The results showed that solvent, catalyst, temperature, and degradation time significantly affected the antiviral activity of the products. In order to indentify the antiviral active compounds, the degradation products were extracted with ethyl acetate (EtOAc), methanol (MeOH), and dimethyl sulfoxide (DMSO) sequentially. The MeOH fraction, which inhibited EMCV strongly, was further separated into subfractions by silica gel chromatography. Structure of the subfraction with high viral inactivation activity was analyzed by gel permeation chromatography (GPC), 2D-HSQC, 31P NMR, and thioacidolysis. It was found that carbohydrate chains attached to the lignin played a critical role in the antiviral activities. When the subfraction was enzymatically hydrolyzed with hemicellulase, the viral inhibition activity was decreased.