Three Monoterpenoids Eucommiol from The Leaves of Eucommia Ulmoides Protect against Neurotoxicity in PC12 Cells

摘要

One new monoterpenoid compound 4'-acetyl-eucommiol (1) and two known compounds eucommiol (2), 1-deoxyeucommiol (3) were isolated from the leaves of Eucommia ulmoides. The structures of the new monoterpenoid eucommiol were elucidated by means of chemical evidence and 1D-and 2D-NMR (~1H, ~(13)C, HSQC, HMBC, ~1H-~1H COSY and NOESY) spectroscopic analysis and HR-ESI-MS. We also investigated the protective effect of compounds 1-3 on the neurotoxicity of PC12 cells induced by amyloid-beta (Aβ_(25-35)), respectively. As a result, compounds 1 and 2 were seen to afford protection against Aβ-induced toxicity in PC12 cells.