Improved Stabilities of Lipase by Immobilization on Eupergit C

摘要

Lipase is frequently used for catalyze wide non-natural substrates in order to obtain enantio- and regioselectivie substrates. This study evaluates the stabilities of lipase by immobilization on Eupergit C. The immobilized lipase had improved stability at 60 ° C for 60 h compared to the free lipase 1 h at 60 ° C. Immobilization resulted in an increase in pH stability over a range of 7.0 - 9.0 and about 54 days for half-life of storage at 4 ° C. The tolerance of lipase to organic solvents was also improved by immobilization, and the immobilized lipase showed activating activity when exposed to hydrophobic solvents.