Site-Specific Conjugation: TG-ADC Platform

摘要

Bacterial Transglutaminase (BTG) allows conjugation of chemical moieties on the endogeneous Q295 from an aglycosylated antibody. We previously demonstrated that BTG two-step process leads to ADCs with DAR of 2 or 4 for antibodies with N297S or N297Q single point mutation respectively. Moreover, this two-step process requires a minimal amount of toxin, which can be a crucial argument with regard to large-scale production of ADCs.