Determination of Clenbuterol and Florfenicol in Milk Using Miniaturized Solid-Phase Extraction Based on Hybrid Material

摘要

A novel sol-gel hybrid material was synthesized based on sol-gel method using TEOS as precursors, KH570 as modifying agent, and EGDMA as cross linking agent. The obtained hybrid material exhibited excellent affinity to clenbuterol and florfenicol, and successfully applied to the home-made miniaturized SPE device for extraction and clean-up of clenbuterol and florfenicol from milk samples. Under the optimized conditions, the consumption of the hybrid material as SPE sorbent was low to 20 mg. Satisfactory linearity ranging from 0.2-20 μg/mL were obtained with r~2≥0.9990, and the recoveries at three spiked levels were ranged from 90.7-103.7% with RSD less than 2.7%.