Preparation and Evaluation of Translucent Balsa-Caffeic Acid Modified Lysozyme Dressing

摘要

objective: using caffeic acid as modifier, dopamine adhesive prepared modified lysozyme, using bacteriostatic circle diameter, bacteria culture and the minimum bacteriostasis concentration bacteriostatic function, the optimal PH, temperature, time, contrast enzymology properties, and hydrophobicity and secondary structure content determination. Methods: the preparation of translucent Balsa, respectively designed for Control group (Control), wood group (A), translucent Balsa group (B), translucent Balsa - lysozyme group (C), translucent Balsa - Caffeic acid modified lysozyme group (D). Results :(1) the bacteriostatic effect and the minimum inhibitory concentration of the Caffeic acid-modified lysozyme> Caffeic acid; The surface hydrophobicity index of the three substances and the stability of the secondary structure were all caffeic acid - modified lysozyme> lysozyme> Caffeic acid; PH= 6, the temperature is 50°C, the response time of 30 min strongest Caffeic acid modified lysozyme activity; (2) the Drug loading and encapsulation rate of group D were better than that of group C. In terms of the release curve, the release of C and D groups in the 48h group tended to be stable and maximum, and the cumulative release percentage of 72h was 85.2% and 90.5% respectively. (3) in the control group, the growth of staphylococcus aureus and e. coli was basically in line with the normal trend, and the antibacterial activity of e. coli and staphylococcus aureus in each group was D>C>B (P<0.05). And 1-7 days, different groups had no value inhibition on fibroblasts (P>0.05); this study successfully optimized the design of modified lysozyme to prepare Translucent Balsa-Caffeic Acid Modified, which has strong antibacterial ability, stable and persistent release, and no cytotoxicity.