The Role of Moringa oliefera-Albumin Combination As A Traditional Medicine to Control The Development of SDF-1 and TER-119~+VLA-4~+ in Diabetes Mice Models

摘要

Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia. Hyperglycemia in DM leads to bone marrow dysfunction in the hematopoiesis process of hematopoietic stem cells (HSC). The objective of this research was to analyze the advantage of Moringa oliefera-Albumin (MA) combination to control the development of SDF-1 and TER-119~+VLA-4~+ in bone marrow. Streptozotocin was used to induce diabetes in BALB/c mice. MA was orally administered during 14 days at dose 1 (50% M: 50% A), dose 2 (75% M: 25% A) and dose 3 (25% M: 75% A). Bone marrow cells from mice were isolated on day 15 after treatment, and the profile of SDF-1 and TER-119~+VLA-4~+ cells were analyzed by flow cytometry. The data were statistically analyzed with one way ANOVA (p < 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that all the doses of MA combination could significantly decrease the development of SDF-1 while only dose 2 (75% M: 25% A) effective to increase the development of TER-119~+VLA-4~+. In conclusions, MA combination has a role in controlling the development of SDF-1 and TER-119~+VLA-4~+ at certain dose. Based on the results, we assumed that MA might be one of a good candidate as therapeutic agent for diabetes mellitus.