Analysis of Competing Endogenous RNA Network and Prediction of Prognosis in Acute Lymphoblastic Leukemia Patients of Phase II and III

摘要

In this paper, we focused on the competing endogenous RNA (ceRNA) of IncRNA-mRNA-miRNA regulatory network and the target mRNAs as biomarker predicting progression of acute lymphoblastic leukemia (ALL) based on the Therapeutically Applicable Research to Generate Effective Treatment (TARGET) database. By screening the RNA-seq, 112 differentially expressed IncRNAs (DElncRNAs), 73 differentially expressed miRNAs (DEmiRNAs) and and 204 differentially expressed mRNAs (DEmRNAs) were found by edgeR package (adjusted P-value < 0.01 and |LogFoldChange| > 4). Among then, the regulatory of 12 DElncRNAs, 5 DEmRNAs and 15 DEmiRNAs was found for the construction of the ceRNA network. 3 DEmRNAs in the ceRNA network, PRKAA2, FOXF2 and TFAP2C, constructed a COX model for the progressive prediction of ALL and was verified by the receiver operating characteristic (ROC) curve, area under the ROC curve (AUC) and kaplan-meier survival curve in a 5-year analysis.