Simulation Analysis of the Homologous Recombination Repair Distribution over the Cell Cycle

摘要

Double-strand breaks (DSBs) are repaired with the use of several distinct mechanisms. The most important of them are nonhomologous end joining (NHEJ) and homologous recombination (HR). These mechanisms have different requirements and are characterized by different repair kinetics. Moreover, HR is restricted to S and G2 phases of the cell cycle, however it is still not clear how percentage of DSBs repaired by HR changes over the cell cycle. In this study we are trying to find the most suitable function describing participation of HR among other types of repair. Using our mathematical model, we simulate the response of average cell treated with ionizing radiation (IR) during G1 phase of the cell cycle. Our results show that the exact shape of the function describing percentage of HR is not as important as the fact that this function should be gradually increasing until at least half of the S phase.