The growth curve of Synechococcus HS-7 and Synechococcus HS-9 isolated from Indonesia hot springs grown in CT medium with initial pH 9

摘要

Cyanobacteria or blue-green algae is a major group of microorganisms containing chlorophyll and phycocyanin pigments. The genus Synechococcus is an example of cyanobacteria. Research on comparison of the growth curve of Synechococcus HS-7 and Synechococcus HS-9 were grown at temperature 35 °C and initial pH of medium 9 had been performed. Synechococcus used in the study is Indonesian indigenous microorganisms. Synechococcus HS-7 isolated from Ciseeng hot springs in West Java with water temperature between habitats was 43°C and pH ranges were 7.5-8. Synechococcus HS-9 isolated from Rawa Danau Banten hot springs with water temperature of habitats was 50 °C and pH ranges were 6.5-7.5. The purpose of this research is to know the differences of the growth curve of Synechococcus HS-7 and Synechococcus HS-9 with the mean of cell density parameter. Counting cell density was done for 7 days from day-0 (t_0) to day-7 (t_7). Each treatment carried out in two replications. The result showed that there were differences between cell density of Synechococcus HS-7 and Synechococcus HS-9. All of Synechococcus strains experienced adaptation (lag) phase and exponential (log) phase. Synechococcus HS-7 experienced adaptation (lag) phase shortly than Synechococcus HS-9. Adaptation (lag) phase in Synechococcus HS-7 occurred at t_0-t_1 while in Synechococcus HS-9 occurred at t_0-t_3. Exponential (log) phase in Synechococcus HS-7 also occurred faster at t_1-t_4. Synechococcus HS-9 experienced exponential (log) phase from day-3 (t_3) until the last day of observation. The highest cell density of Synechococcus HS-7 occurred at t_6 while Synechococcus HS-9 occurred at t_7 for 7 days observation. Synechococcus HS-9 grows well in CT medium with initial pH 9 than Synechococcus HS-7.