3D Engineering of Synthetic Tumors

摘要

Conflicting data from in vitro,in vivo,and clinical studies exist regarding the magnitude of the contribution of therapeutic agents in disease treatment,particularly in the treatment of most forms of cancer for which the long-term risk of disease relapse is-30%.Although much of our understanding on how cells divide,migrate,and die have been generated by studying cells on two-dimensional(2D)surfaces;processes such as morphogenesis,tissue remodeling,and metastasis alter the 3D organization of the microenvironment itself making it difficult,if not impossible,to recapitulate these processes in 2D culture systems.To more closely capture the conditions that cells experience in vivo,we have developed a 3D sacrificial printing system to generate biodegradable scaffolds of filament networks to grow spheroid-printed cells in a defined architectural matrix and keep them viable for long periods while allowingreal-time bioluminescence recording.