Cow's Milk Yogurt Modulate Expression of STAT5, BMI-1 and LGR-5 in Ileum of Indomethacine-Induced Inflammatory Bowel Disease (IBD) in Rats.

摘要

Inflammatory bowel disease (IBD) is characterized by a chronic recurrent inflammation of the gastrointestinal tract. The environmental factors affecting IBD include diet, the use of antibiotics and nonsteroidal anti-inflammatory drugs (NSAIDs) and stress. All of which can directly or indirectly affect the epithelial barrier of intestinal that consists of the intestinal epithelial cells (IEC). The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) 3 pathway is critically involved in IEC proliferation during homeostasis as well as enhance proliferation that needed for tissue repair in active IBD. Mucosal damage triggers Upd3 cytokin to activate JAK/STAT Pathways in renewing epithelium. Activation of the stem cells characterized by expression of LGR5+ and BMI-1. Effect of cow's milk yogurt on the expression of STAT5, BMI-1 and LGR5+ in ileum of Indomethacine-Induced IBD rats (Rattus norvegicus) were studied. The IBD rats were then orally administrated with cow's milk yogurt in daily dose of 300, 600 and 900 mg/kg body weight for 14 days. The effect of yogurt treatment on Stat5, BMI-1 and LGR-5 of rats ileum were evaluated using immunohistochemistry method. The results showed that the expression of Stat5, BMI-1 and LGR-5 increased significantly after yogurt administration. Structurally also detected there were regeneration of the epithelial cells and repairing the epithelial damage. The current study suggests that cow's milk yoghurt treatment was able to regulate stem cell proliferation and repair intestinal damage in Indomethacine-Induced IBD rats model.