Antioxidant Activity Assay of Alpha-mangostin for Amelioration of Kidney Structure and Function in Diabetic Mice

摘要

This research was aimed to investigate the ability of a alpha-mangostin compound derived from mangosteen pericarp to reduce plasma blood urea nitrogen (BUN) and creatinine level, and also to ameliorate the damaged renal proximal tubular of the kidneys in diabetic mice. An in-vivo antioxidant test was conducted using male BALB/c mice. The mice were divided into two groups; normal control (KN) and streptozotocin-induced diabetic mice. Streptozotocin (STZ) induction was performed using multiple low-doses of 30 mg/kg injected for five consecutive days. The diabetic mice were separated again into three sub-groups; diabetic control (KD), diabetic mice treated with Metformin HCl (KM), and diabetic mice treated with alpha-mangostin. The alpha-mangostin treatment group was categorised based on the dose given; P1 (2 mg/kg body weight), P2 (4 mg/kg body weight) and P3 (8 mg/kg body weight). Before and after STZ injection, the body weight and fasting blood glucose were measured. The body weight and fasting blood glucose were measured on the 1st, 7th, and 14th day of alpha-mangostin treatment. Alpha-mangostin was given orally for 14 days. On the 15th day, the plasma BUN and creatinine level were measured using Pentra C 200. Interestingly, the administration of alpha-mangostin was able to lower the plasma BUN and creatinine level, and also the ameliorate-damaged renal proximal tubular epithelial cells of the kidney in diabetic mice leseened significantly.