A SELECTIVE AND HIGHLY SENSITIVE ANALYTICAL METHODOLOGY FOR DETERMINATION OF BISPHENOL A IN CANNED TOMATOES

摘要

A selective and highly sensitive analytical methodology for determination of Bisphenol A (BPA) in canned tomatoes was developed and validated. The method was based on selective liquid/solid extraction, combined with liquid chromatography-electrospray ionization tandem mass spectrometry in the multiple reaction monitoring (MRM) mode and negative ionization. The linearity of the detector response was verified for canned tomato samples and the limit of quantification of the method (LOQ) was calculated to be 0.02 μg/Kg. The method was in-house validated evaluating specificity, trueness, within-day and between-days precision. The mean recoveries of BPA from tomato samples were higher than 94% and good reproducibility was observed. The matrix effect was studied and compensated using deuterated labeled standard (d_6-BPA). To assess the BPA migration from epoxy-coating cans, 16 samples belonging to six commercial brands, retailed in Italian markets, were tested. All the tomato samples exhibited migration levels below 4 μg/Kg. In any case no sample contained BPA exceeding the European Union limit for migration (600 μg/Kg of food).