Study of adsorption and preparation of ginsenoside Rg1 molecularly imprinted monolithic column

摘要

The molecularly imprinted monolithic column (MIMC) was synthesized using a-methacrylic acid as functional monomers, ethylene glycol dimethacrylate as cross-linker, the mixture of isopropanol and dodecadol as porogenic reagent and Ginsenoside Rg1 as template molecules by in-situ polymerization. The performances of separation and adsorption of MIMC were investigated, the results of research indicated that optimal mobile phase was methanol-acetate (0.0lmol/L, PH=3.5) with a volume ratio of 40:60, separation factor 1.769, langmuir produced a very good fit, and the specific adsorption sites had formed to ginsenoside Rg1 by frontal chromatography, saturated adsorption capacity was 0.248mg/g. MIMC has special molecule recognition ability to Rg1. The result of scanning electron microscopy (SEM) showed that the unique porous structure was formed in the polymer, which was beneficial to the flow of mobile phase and the mass transfer of template molecules.