Lipase from Fusarium heterosporum expressed in Ogataea polymorpha

摘要

At the request of the Codex Committee on Food Additives at its Forty-sixth Session (FAO/WHO, 2014), the Committee evaluated the safety of lipase (triacylglycerollipase; Enzyme Commission No. 3.1.1.3) from Fusarium heterosporum expressed in Ogataea polymorphs which it had not previously considered. Ogataea polymorpha was recently renamed from Hansenula polymorpha based on genetic analyses (Suh & Zhou, 2010). Lipase hydrolyses ester bonds in the 1- and 3-positions of fatty acids in triglycerides. The enzyme also has activity towards sn-1 ester bonds in other lipid components, including diacyl-phospholipids and diacyl-galactolipids. In this report, the expression"lipase" refers to the lipase enzyme and its amino acid sequence, the expression "lipase liquid enzyme concentrate" refers to the test material used in the toxicity studies evaluated, and the expression "lipase enzyme preparation" refers to the preparation formulated for commercial use. The lipase enzyme preparation is used as a processing aid in the manufacture of bakery products, pasta and noodles, in egg yolk and in the degumming of edible oil.