Organic Nanobiomimetic Memristive/Memcapacitive Devices Ultrasensitive Direct Detect Matrix Matelloproteinase-2 in Human Serum

摘要

Matrix Matelloproteinase-2 (MMP-2) plays a key role in many diseases. A new type of dual-functioning device was developed for fast, direct ultrasensitive detection of MMP-2 using NIST SRM965A reference human serum specimens under label-free, probe-free and reagent-free conditions. The organic memcapacitive/memristive device was based on a self-assembled polymer membrane having polarized 3D array crossing-nanotube structures on a gold substrate that enables the selective inducement of bio-communication with MMP-2. The characteristics of memcapacitor/memristor were observed in the i-V curves of the hysteresis. The Chronoamperometry (CA) method was used with a Detection of Limits (DOL) value of 8.67×10~(-18)g/mL in PBS solution related to current density between 1.47 μA/cm~2 and 919.2 mA/cm~2 over MMP-2 concentration between 20 ag/mL to 100 ng/mL with a Relative Pooled Standard Deviation 1.4% (n=26). The Double Step Chronopotentiometry (DSCPO) method produced a similar impression value of 1.47% (n=18) over MMP-2 concentration 40 ag/mL to 100 ng/mL over energy density between 185 - 0.47 μWHR/cm~3. The sensor was able to direct detect MMP-2 in pure NIST serum specimens in the concentrations of 81.15 ± 0.10 ag/mL for normal glycemia, 1.13 ± 0.0016 pg/mL for hypoglycemia and 1.4± 0.0001 pg/mL for hyperglycemia serum, respectively.