A Mechanistic Study of IncRNA Fendrr Regulation of FoxF1 Lung Cancer Tumor Suppressor

摘要

Long non-coding RNAs are known to play multiple roles in the complex machinery of the cell. However, their recent addition to genomic research has increased the complexity of gene expression analyses. In this work, we perform a computational study that aims to contribute to the current understanding of the mechanisms that underlie the experimentally suggested interaction between the IncRNA Fendrr and FoxF1 lung cancer tumor suppressor in carcinogenesis. Results suggest that there exists indeed a multi-level interaction between Fendrr and FoxF1 promoter region, both direct via RNA-DNA:DNA triplex domain formation or mediated by proteins that interact simultaneously with the promoter region of FoxF1 and Fendrr transcripts. Moreover, the applied computational methodology can serve as a pipeline to process any candidate IncRNA-gene pair of interest and obtain putative sources of IncRNA-gene interaction.