Exploration on the Best Way of Separating Auricularia Auricula' Mother Strains

摘要

3 mother strains were obtained using 3 different separation methods (i.e. spore separation, sporocarp separation, and mushroominhabiting wood separation), respectively. These mother strains were processed to some extent. First, the mycelial growth rates of spore separation and sporocarp separation, were known to be fast, 0.39 cm/d and 0.37 cm/d respectively, but the rate of mushroominhabiting wood separation was the slowest, only 0.32 cm/d, after a statistical analysis. Second, the growth trend of the mycelium obtained using spore separation and sporocarp separation was good, but the mycelium obtained using spore separation was thicker, neater, and whiter, after a comparison. Third, an extracellular enzyme assay found that the enzymatic activity of the strains obtained using spore separation was the best. Finally, an high-temperature resistant test found that the strains obtained using spore separation and sporocarp separation were resistant to high temperature, and thus, it was concluded that spore separation was better than sporocarp separation and mushroominhabiting wood separation to separate the mother strains of auricularia auricula.