Study on the saccharification conditions of fuel ethanol from corn straw

摘要

To study the saccharification conditions of cellulase on lignocellulose material, this paper used corn straw as the material to do research on the effect of cellulase. The hydrolysis factors, including reaction temperature, reaction time and cellulase concentration, were measured in order to determine the optimal saccharification conditions for fuel ethanol production. The results showed that, when the reaction temperature was 45°C, reaction time was 72 h and cellulase concentration was 60 IU/g, the hydrolysis rate can respectively reach 6.25%, 14.7% and 14.4%. By the analysis of orthogonal test, the main factors effects the saccharification was reaction time. And the optimal combination of saccharification conditions was the reaction time of 54 h, the reaction temperature of 45°C, and the cellulase concentration of 55 IU/g.