9-methoxycanthin-6-one Production in Elicited Hairy Roots Culture of Eurycoma longifolia

摘要

Eurycoma longifolia (Tongkat Ali) is a highly sought after medicinal plant in Malaysia. Propagation of E. longifolia through tissue culture has been reported in order to cater the industry demands for planting and raw materials as well as for conservation purposes. E. longifolia hairy roots culture has been developed using Agrobacterium rhizogenes for the production of Tongkat Ali phytochemicals. Effects of three elicitors; methyl jasmonate, salicylic acid, and yeast extract at different concentrations were evaluated on the production of 9-methoxycanthin-6-one in E. longifolia hairy roots. The cultures were elicited at early exponential growth phase, followed by extraction of 9-methoxycanthin-6one using methanol and HPLC analysis. Elicitation with methyl jasmonate at all concentrations increased 9methoxycanthin-6-one up to 1- 3 fold and treatment with (0.1 mM) was most efficient in enhancing 9-methoxycanthin-6one production up to 3.902 mg/g dry weight after 7 days (168 hours) elicitation.