Effects of Antioxidant Compounds on Methamphetamine-Induced Learning and Memory Impairment in Mice

摘要

Purpose: To observe effects of antioxidant compounds on the learning and memory impairment induced by methamphetamine (MA) in mice. Methods: Fifty 6 weeks old male C57bl/6 mice were randomly divided into 5 groups: group A was control group, group B was model group, group C was 50 mg/kg group, group D was 100 mg/kg group and group E was 200 mg/kg group. Groups B, C, D, and E were intraperitoneally injected with methamphetamine to prepare a model of learning and memory impairment in mice, and group A was injected with saline. Groups C, D, and E were intragastrically pre-administered with 50, 100, and 200 mg/kg of antioxidant compound for 2 weeks before modeling, and continued to be administered during modeling and behavioral testing, and groups A and B were administered with saline. The Morris water maze was used to test the short-term learning and memory ability of mice (the swimming speed, escape latency, percentage of time spent in the target quadrant). Determination of superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) expression levels in serum, hippocampus and striatum of mice by enzyme-linked immunosorbent assay (ELISA). Results: Behavioral test results: Compared with group A, there was no significant difference in the average speed between days 1-5 of group B, the escape latency was significantly increased on days 3 and 5 (P<0.05), and the percentage of time spent in target quadrant decreased significantly (P<0.05); compared with group B, there was no significant difference in the average speed and escape latency between days 1 -5 of groups C, D and E, but the percentage of time spent in target quadrant of group D increased significantly (P<0.05). ELISA results: in serum, compared with group A, SOD and T-AOC of group B were significantly decreased, and MDA of group B was significantly increased (P<0.05); compared with group B, SOD of groups D and E were significantly increased, T-AOC of groups C and D were significantly increased, and MDA of group D was significantly decreased (P<0.05). In the hippocampus, compared with group A, SOD and T-AOC of group B were significantly decreased, and MDA of group B was significantly increased (P<0.05); compared with group B, SOD and T-AOC of group D were significantly increased, and MDA of group D was significantly decreased (P<0.05). In the striatum, compared with group A, the SOD of group B was significantly decreased, and MDA of group B was significantly increased (P<0.05); compared with group B, SOD of groups C and D were significantly increased, and MDA of groups C, D and E were significantly decreased (P<0.05). Conclusion: The 100mg/kg antioxidant compounds could improve the oxidative stress of mice induced by MA, which could alleviate the memory damage in mice.