Microbiological distribution of multi-level-well samples in the Coast of South China

摘要

In this study, the Microbiological communities were investigated by culture-independent molecular approaches. Four 16S rRNA gene libraries from mineral water and different groundwater of wells collected from Zhuhai of the South China were constructed and analyzed by amplified ribosomal DNA restriction analysis technique (ARDRA). The clones of dominant ARDRA patterns were selected to sequence. The phylogenetic analysis indicated that the sequences from dominant ARDRA patterns belonged to Proteobacteria, Bacteroidetes , archaea ,Actinobacteria, Planctomycetes, and Candidate division OP3, OP8, OP11 et al. The percentages of Proteobacteria isolated from multi-level-well samples D1,D5,D6 were respectively 32.3 % ,34.3%,46.7%;The Bacteroidetes were respectively 5.0 % ,11.2 % ,6.5 %; and Actinobacteria were respectively 5.4%,6.6%,7.8%. As a background, the seawater clone library, with the majority (32.8%) of the clones clustering within the Proteobacteria, the second most abundant group were Bacteroidetes and Planctomycetes (both 9.3%), The results suggest that microbial community from different vertical layers of groundwater in pristine habitats that have been described for the first time in China.