Optimization of Culture Media and ITS Identification of Tricholoma matsutake

摘要

Aims: To optimize the culture media of 0288 Tricholoma matsutake and to identify it according to its rDNA ITS sequence. Methods: Fruit body, collected from Heilongjiang province, is used as the material, and the mycelium is isolated and radiated under ultraviolet. Then the radiated mycelium is cultured in five different media and its morphology of fungus colony and growth rate are investigated. After that, the rDNA ITS sequences of 0288 and the natural fruit body of T. matsutake are analyzed and identified by PCR and sequencing. Results: The radiated mycelium grew faster in formula A culture medium than others and its average growth rate is up to 0.575mm/d. Results also showed that the homology of the rDNA ITS sequence is almost up to 99% between 0288 and the natural fruit body. Through this experiment, the best growth condition of 0288 is obtained and its fruit body is induced as pure cultural product. It would lay strong foundation for the artificial domestication of the wild T. matsutake.