Modification of PK-15 Cells Proteome in the early stage of Classical Swine Fever Virus Infection

摘要

To uncover host cellular responses in the early stage of classical swine fever virus infection, a proteomic analysis was conducted using 2D electrophoresis followed by MALDI-TOF-TOF identification. Altered expression of 21 protein spots in infected pk-15 cells at 24 h p.i. were identified in 2D gels, with 13 of these being characterized by MALDI-TOF-TOF. These proteins function in cytoskeletal, energy metabolism, nucleic acid/processing, and cellular stress. The expression alteration of these proteins presents the changes in physiological functions of host cells and provides a clue for further understanding of the mechanisms of CSFV infection and pathogenesis.