Monoclonal antibody for Classical Swine Fever Virus CSFV, hybridoma cell line producing the Kit for detecting Classical Swine Fever Virus CSFV using the monoclonal antibody for CSFV

摘要

PURPOSE: A monoclonal antibody against classical swine fever virus (CSFV), a hybridoma cell line producing the same, and a method for detecting CSFV using the same are provided, thereby significantly reducing the time for detecting the classical swine fever virus (CSFV), and improving the accuracy of the detection. CONSTITUTION: The monoclonal antibody against classical swine fever virus (CSFV) is produced from the hybridoma cell line CSFV-LOM 1(KCLRF-BP-00069) or hybridoma cell line CSFV-LOM 2(KCLRF-BP-00070). A conjugate of peroxidase and monoclonal antibody against CSFV is provided. The method for detecting CSFV comprises the steps of: (1) diluting the monoclonal antibody against CSFV in a coating buffer solution, spotting the diluted solution on ELISA plate, and adsorbing it; (2) cleaning the ELISA plate to remove unadsorbed monoclonal antibody; (3) reacting a feces sample with the plate; (4) cleaning the plate to remove the feces sample unbound by the monoclonal antibody for coating; (5) reacting the monoclonal antibody for detecting with an enzyme conjugate; (6) cleaning the plate to remove unbound conjugate; and (7) adding a substrate into the plate and measuring the absorbance by the chromogenic reaction.