Preparation and Evaluation of the Pegylated Cerebrolysin

摘要

Four kinds of brain active peptide (BAP) in Cerebrolysin were effectively modified by mPEG-ALD_(5000) of single molecule. At pH = 5.0,25°C, with Cerebrolysin (injection)/mPEG-ALD_(5000) feed ratio of 5 mL : 1 g, the modification rate reached 72.805% after 30 min. The method of nerve cells culture in vitro was used to evaluate the biological activity of modified Cerebrolysin. The result showed that after the BAPs in Cerebrolysin were modified by mPEG-ALD_(5000), the activity of promoting nerve cell growth of Cerebrolysin was significantly improved.