Antioxidant activities of ethanol extracts from Zanthoxylum avicennae

摘要

The Zanthoxylum avicennae 95% EtoH extracts after concentration were dissolved in H_2O and extracted with EtoAc and n-BuOH Successively. The n-BuOH layer was added to Diaion HP-20 macroporous resin column, then the resin was washed by distilled water to get rid of impurity, then washed by 20%, 40% and 60% methanol individually, and obtained M20, M40 and M60 respectively. And antioxidant activity was assessed using three methods: DPPH radical-scavenging activity, ABTS+ radical-scavenging activity, ferric reducing antioxidant power (FRAP) assay. The results indicate that TEAC value of M40 were higher than other part extracted from Zanthoxylum avicennae 95% EtoH extracts during three methods. The results of the experiments also can find M40 is the strongest antioxidant activity part in Zanthoxylum avicennae 95% EtoH extracts and provide reference for further isolating the part M_(40).