Study on Toxicity of Copper Oxide Engineered Nanoparticles to Tobacco BY-2 Cells

摘要

The extensive application of nanoparticles will inevitably lead them to be released into the environment, which causes potential hazard to the ecosystem. This study was to provide the information of the toxicity of CuO ENPs to BY-2 cells in vitro. Tabacco BY-2 cells were exposed to 4 mg/L and 12 mg/L CuO ENPs. Cell viability was evaluated in 0 h, 2 h, 4 h, 8 h, 12 h, 16 h, 20 h and 24 h. The viability of BY-2 cells exposed to CuO ENPs gradually decreased with exposure time and the exposure concentration increasing. After 24 hours exposure to 12 mg/L CuO ENPs, plasmolysis occurred in cells. Some ENPs were absorbed on the cell wall, and only a small number of ENPs entered the cell. Membrane leakage turned up. This might be caused by lipid peroxides, destroying biological membranes.