Determination of doxycycline residues in milks and content in serum,tablet and injection samples with self-ordered ring fluorescence microscopic imaging technique

摘要

A new method of determinating doxycycline residues in milks and content in serum,table and injection samples was established with Self-ordered ring fluorescence microscopic imaging technique. In the presence of hexahydropyridine and poly (vinyl alcohol)-124, Zn~(2+)-doxycycline (DC) system can form a SOR on the hydrophobic glass slides surfaces based on the capillary effect. The maximum fluorescent intensity (I_(max)) at central ring belt was found to be proportional to DC content. when the droplet volume is 0.5μL, the present SOR method can be used to determine DC in a range of 4.54×10~(-15)-6.81×10~(-13) mol?ring~(-1), and the limit of detection (LOD) with a threefold signal to noise ratio (S/N = 3) was 4.54×10~(-15) mol?ring~(-1) (2.17×10~(-8) mol?L~(-1)). With the present method, the residues in milks and content in serum, tablet and injection samples were satisfactorily detected with recoveries of 96.3-102.0% and RSD of 1.4-2.2%, respectively, indicating that the method is reliable and practical.